Rapid hybrid de novo assembly of a microbial genome using only short reads: Corynebacterium pseudotuberculosis I19 as a case study

► We describe an integrated approach for de novo assembly using only short reads. ► We combine De Bruijn graph and Overlap Layout Consensus methods. ► Showing an approach without the use of costly and time-consuming benchwork. ► Presenting whole genome sequence of Corynebacterium pseudotuberculosis I19.