کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10903834 | 1086530 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
MiR-21 inhibits autophagy by targeting Rab11a in renal ischemia/reperfusion
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کلمات کلیدی
AKIAutophagosomeRab11aphagophore assembly siteI/R - I / Racute kidney injury - آسیب حاد کلیهAutophagy - اتوفاژیRenal ischemia/reperfusion - ایسکمی / مجاری کلیوی کلیویischemia–reperfusion - ایسکمی-رپرفیوژنmiR-21 - به miR-21COPD - بیماری مزمن انسدادی ریهChronic obstructive pulmonary disease - بیماری مزمن انسدادی ریهPAS - نه
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Renal ischemia-reperfusion (I/R) is one of the main causes of the acute kidney injury (AKI) that usually occurs during clinical surgery. Autophagy plays an important role in recovery from acute ischemic kidney injury. MicroRNA-21 (miR-21) was reported to inhibit autophagy in several diseases. However, the molecular mechanism of miR-21 on autophagy during renal I/R is still unclear. For the in vitro study, NRK-52E cells were transfected with miR-21 mimics and subjected to I/R. Results showed that miR-21 mimics inhibited cell viability and induced cell apoptosis in NRK-52E cells. Expression of beclin-1 and LC3-II was induced, and p62 was decreased by I/R. miR-21 mimics inhibited this induction. RT-PCR and western blotting assay showed that miR-21 mimics inhibited the protein level of Rab11a, but not the mRNA level. A luciferase activity assay showed that miR-21 directly targeted Rab11a 3â²-UTR. Rab11a overexpression attenuated the effect of miR-21 mimics and I/R on cell viability and cell apoptosis. The expression of beclin-1 and LC3-II was increased, and p62 was decreased by Rab11a overexpression. For the in vivo assay in a rat I/R model, the miR-21 level was increased during renal I/R injury. Pre-treatment with miR-21 inhibitor injection attenuated the renal injury, and enhanced expression of LC3-II and beclin-1. The results showed that miR-21 inhibited autophagy by targeting Rab11a in renal I/R, indicating that Rab11a might be a new medical target for the treatment of renal I/R.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 338, Issue 1, 15 October 2015, Pages 64-69
Journal: Experimental Cell Research - Volume 338, Issue 1, 15 October 2015, Pages 64-69
نویسندگان
Xiujuan Liu, Quan Hong, Zhen Wang, Yanyan Yu, Xin Zou, Lihong Xu,