کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904017 | 1086551 | 2014 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sphingosine-1-phosphate mediates epidermal growth factor-induced muscle satellite cell activation
ترجمه فارسی عنوان
اسپینوزین-1-فسفات عامل فعال سازی سلول ماهواره ای ناشی از فاکتور رشد اپیدرمی است
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کلمات کلیدی
EGFEDGC2C12SphKDulbecco׳s modified Eagle mediumPDGFEDLS1PTBSTPKCGPCRDMSERKPI3KPBSDMEMIGFSDSFBSJnkBSA - BSADMSO - DMSOG-protein coupled receptor - G-پروتئین همراه گیرندهMAPK - MAPKSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAbovine serum albumin - آلبومین سرم گاوSphingosine kinase - اسپینوزین کینازSphingosine-1-phosphate - اسپینگسین-1-فسفاتextensor digitorum longus - اکستانسور بلند شست انگشتانBrdU - بروموداکسی اوریدینDimethyl sulfoxide - دیمتیل سولفواکسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوSatellite cells - سلول های ماهواره ایReserve cells - سلولهای ذخیره شدهepidermal growth factor - عامل رشد اپیدرمیSkeletal muscle - عضله اسکلتیplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتInsulin-like growth factor - فاکتور رشد مانند انسولینphosphoinositide 3-kinase - فسفینوزیتید 3-کینازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریProtein kinase C - پروتئین کیناز سیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenPolyvinylidene fluoride - پلی وینیلیدین فلورایدEndothelial differentiation gene - ژن تمایز اندوتلیالextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
Skeletal muscle can regenerate repeatedly due to the presence of resident stem cells, called satellite cells. Because satellite cells are usually quiescent, they must be activated before participating in muscle regeneration in response to stimuli such as injury, overloading, and stretch. Although satellite cell activation is a crucial step in muscle regeneration, little is known of the molecular mechanisms controlling this process. Recent work showed that the bioactive lipid sphingosine-1-phosphate (S1P) plays crucial roles in the activation, proliferation, and differentiation of muscle satellite cells. We investigated the role of growth factors in S1P-mediated satellite cell activation. We found that epidermal growth factor (EGF) in combination with insulin induced proliferation of quiescent undifferentiated mouse myoblast C2C12 cells, which are also known as reserve cells, in serum-free conditions. Sphingosine kinase activity increased when reserve cells were stimulated with EGF. Treatment of reserve cells with the D-erythro-N,N-dimethylsphingosine, Sphingosine Kinase Inhibitor, or siRNA duplexes specific for sphingosine kinase 1, suppressed EGF-induced C2C12 activation. We also present the evidence showing the S1P receptor S1P2 is involved in EGF-induced reserve cell activation. Moreover, we demonstrated a combination of insulin and EGF promoted activation of satellite cells on single myofibers in a manner dependent on SPHK and S1P2. Taken together, our observations show that EGF-induced satellite cell activation is mediated by S1P and its receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 326, Issue 1, 1 August 2014, Pages 112-124
Journal: Experimental Cell Research - Volume 326, Issue 1, 1 August 2014, Pages 112-124
نویسندگان
Yosuke Nagata, Kazuya Ohashi, Eiji Wada, Yuki Yuasa, Masataka Shiozuka, Yoshiaki Nonomura, Ryoichi Matsuda,