کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10905474 | 1086756 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transient expression of laminin α1 chain in regenerating murine liver: Restricted localization of laminin chains and nidogen-1
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کلمات کلیدی
BECsPBS (−)SECSCK19PECAMFBSDMEMBSA - BSADulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoPHx - PHXbovine serum albumin - آلبومین سرم گاوHepatic regeneration - بازسازی کبدfetal bovine serum - سرم جنین گاوSinusoidal endothelial cells - سلولهای اندوتلیال سینوسbiliary epithelial cells - سلولهای اپیتلیال صفراویcytokeratin 19 - سیتوکراتین 19Basal lamina - لامین BasalLaminin - لامینینplatelet endothelial cell adhesion molecule - مولکول چسبندگی سلول اندوتلیال پلاکتElectron microscopy - میکروسکوپ الکترونی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Most interstitia between epithelial and endothelial cells contain basal laminae (BLs), as defined by electron microscopy. However, in liver, the sinusoidal interstitium (called space of Disse) between hepatocytes and sinusoidal endothelial cells (SECs) lacks BLs. Because laminins are major components of BLs throughout the body, whether laminins exist in sinusoids has been a controversial issue. Despite recent advances, the distribution and expression of laminin chains have not been well defined in mammalian liver. Here, using a panel of antibodies, we examined laminins in normal and regenerating mouse livers. Of α chains, α5 was widely observed in all BLs except for sinusoids, while the other α chains were variously expressed in Glisson's sheath and central veins. Laminin γ1 was also distributed to all BLs except for sinusoids. Although the β2 chain was observed in all BLs and sinusoids, the expression of β1 chain was restricted to Glisson's sheath. Detailed analysis of regenerating liver revealed that α1 and γ1 chains appeared in sinusoids and were produced by stellate cells. The staining of α1 and γ1 chains reached its maximum intensity at 6 days after two-thirds partial hepatectomy (PHx). Moreover, in vitro studies showed that α1-containing laminin promoted spreading of sinusoidal endothelial cells (SECs) isolated from normal liver, but not other hepatic cells. In addition, SECs isolated from regenerating liver elongated pseudopodia on α1-containing laminin more so than did cells from normal liver. The transient expression of laminin α1 may promote formation of sinusoids after PHx.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 305, Issue 1, 15 April 2005, Pages 99-109
Journal: Experimental Cell Research - Volume 305, Issue 1, 15 April 2005, Pages 99-109
نویسندگان
Yamato Kikkawa, Yoichi Mochizuki, Jeffrey H. Miner, Toshihiro Mitaka,