Acylation-dependent and-independent membrane targeting and distinct functions of small myristoylated proteins (SMPs) in Leishmania major

► The small myristoylated protein (SMP)-4 localizes at the pellicular plasma membrane. ► SMP-4 localisation relies on acylation-dependent and -independent sorting signals. ► The C-terminal alpha helices of SMP-1 and SMP-4 contain divergent functions.