کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11028565 1646765 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
UHPLC-MSn-assisted characterization of bioactive alkaloids extracted from Nitraria sibirica leaves and enriched using response surface method and adsorption on macroporous resin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
UHPLC-MSn-assisted characterization of bioactive alkaloids extracted from Nitraria sibirica leaves and enriched using response surface method and adsorption on macroporous resin
چکیده انگلیسی
In Xinjiang, China, Nitraria sibirica Pall. is traditionally used to treat hypertension, menstrual disorders, and gastroenteritis. Following process optimization by response surface modeling and Box-Behnken analysis, we extracted 3.83 mg/g of alkaloids from N. sibirica leaves in two cycles of extraction for 2.38 h in 89.45% ethanol at liquid-solid ratio 11.381 (mL/g). Subsequent adsorption on HPD-450 resin further enriched the total alkaloid content to 18.08%. Subsequently, 28 compounds, including 15 alkaloids and 13 flavonoids and phenolic compounds, were identified in the extract by UHPLC-Q-Orbitrap-MS. Strikingly, the extracts were strongly antimicrobial againstCandida albicans and Staphylococcus aureus, and significantly antiproliferative against A549, MCF7, and Hela tumor cells, probably due to induction of apoptosis. Finally, the extracts efficiently scavenged radicals with IC50 50.90 ± 6.45 μg/mL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 125, 1 December 2018, Pages 529-536
نویسندگان
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