کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1910641 | 1046780 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Chemical model systems for cellular nitros(yl)ation reactions
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کلمات کلیدی
Spermine NONOatePEG-SODICDHS-nitrosylationGSHSIN-1GSNODHRS-nitrosoglutathioneXanthine oxidase - زانتین اکسیدازIsocitrate dehydrogenase - ایزوسیترات دهیدروژنازdihydrorhodamine 123 - دی هیدرو رودامین 123Diaminonaphthalene - دیامینونافلینFree radicals - رادیکال آزادSuperoxide - سوپر اکسیدCu,Zn-SOD - مس، روی-SODCopper, zinc superoxide dismutase - مس، سوپراکسید دیسموتاز رویNitric oxide - نیتریک اکسیدhypoxanthine - هیپوکسانتینDAN - وPeroxynitrite - پروکسی نیتریتGlutathione - گلوتاتیون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
S-nitros(yl)ation belongs to the redox-based posttranslational modifications of proteins but the underlying chemistry is controversial. In contrast to current concepts involving the autoxidation of nitric oxide (NO, nitrogen monoxide), we and others have proposed the formation of peroxynitrite (oxoperoxonitrate (1â)) as an essential intermediate. This requires low cellular fluxes of NO and superoxide (O2â), for which model systems have been introduced. We here propose two new systems for nitros(yl)ation that avoid the shortcomings of previous models. Based on the thermal decomposition of 3-morpholinosydnonimine, equal fluxes of NO and O2â were generated and modulated by the addition of NO donors or Cu,Zn-superoxide dismutase. As reactants for S-nitros(yl)ation, NADP+-dependent isocitrate dehydrogenase and glutathione were employed, for which optimal S-nitros(yl)ation was observed at nanomolar fluxes of NO and O2â at a ratio of about 3:1. The previously used reactants phenol and diaminonaphthalene (C- and N-nitrosation) demonstrated potential participation of multiple pathways for nitros(yl)ation. According to our data, neither peroxynitrite nor autoxidation of NO was as efficient as the 3 NO/1 O2â system in mediating S-nitros(yl)ation. In theory this could lead to an elusive nitrosonium (nitrosyl cation)-like species in the first step and to N2O3 in the subsequent reaction. Which of these two species or whether both together will participate in biological S-nitros(yl)ation remains to be elucidated. Finally, we developed several hypothetical scenarios to which the described NO/O2â flux model could apply, providing conditions that allow either direct electrophilic substitution at a thiolate or S-nitros(yl)ation via transnitrosation from S-nitrosoglutathione.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 47, Issue 4, 15 August 2009, Pages 458-467
Journal: Free Radical Biology and Medicine - Volume 47, Issue 4, 15 August 2009, Pages 458-467
نویسندگان
Andreas Daiber, Stefan Schildknecht, Johanna Müller, Jens Kamuf, Markus M. Bachschmid, Volker Ullrich,