کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1922943 1535844 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The extracellular matrix modulates H2O2 degradation and redox signaling in endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
The extracellular matrix modulates H2O2 degradation and redox signaling in endothelial cells
چکیده انگلیسی


• The extracellular matrix (ECM) regulates H2O2 consumption by endothelial cells.
• Membrane biophysical properties are not affected by the ECM.
• The ECM regulates GPx activity and the extent of PTEN oxidation.
• We propose that the ECM modulates redox-signaling by controlling H2O2 degradation.

The molecular processes that are crucial for cell function, such as proliferation, migration and survival, are regulated by hydrogen peroxide (H2O2). Although environmental cues, such as growth factors, regulate redox signaling, it was still unknown whether the ECM, a component of the cell microenvironment, had a function in this process.Here, we showed that the extracellular matrix (ECM) differently regulated H2O2 consumption by endothelial cells and that this effect was not general for all types of cells. The analysis of biophysical properties of the endothelial cell membrane suggested that this modification in H2O2 consumption rates was not due to altered membrane permeability. Instead, we found that the ECM regulated GPx activity, a known H2O2 scavenger. Finally, we showed that the extent of PTEN oxidation was dependent on the ECM, indicating that the ECM was able to modulate H2O2-dependent protein oxidation.Thus, our results unraveled a new mechanism by which the ECM regulates endothelial cell function by altering redox balance. These results pinpoint the ECM as an important component of redox-signaling.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Redox Biology - Volume 6, December 2015, Pages 454–460
نویسندگان
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