کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1923350 1048886 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Activity of cyclin B1 in HL-60 cells treated with etoposide
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Activity of cyclin B1 in HL-60 cells treated with etoposide
چکیده انگلیسی

Cyclin B1 triggers G2/M phase transition phosphorylating with its catalytical partner − Cdc2 many of the molecular targets essential for cell cycle progression. Human leukemia cell line HL-60 were treated with increasing doses of etoposide (ETP) (0.5; 0.75; 1 μM) to investigate how the drug affects cell morphology, viability, cell cycle distribution and expression of cyclin B1. To achieve this aim we applied light and transmission electron microscopy to observe morphological and ultra structural changes, image-based cytometry for apoptosis evaluation and cell cycle analysis, and then we conducted immunohistochemical and immunofluorescence staining to visualize cyclin localization and expression. Quantitive data about cyclin B1 expression were obtained from flow cytometry. Etoposide caused decrease in cell viability, induced apoptosis and G2/M arrest accompanied by enhanced expression of cyclin B1. Changes in expression and localization of cyclin B1 may constitute a part of the mechanism responsible for resistance of HL-60 cells to etoposide. Our results may reflect involvement of cyclin B1 in opposite processes − apoptosis induction and maintenance of cell viability in leukemia cells. We hypothesized possible roles and pathways by which cyclin B1 takes part in drug treatment response and chemosensitivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 118, Issue 5, June 2016, Pages 537–543
نویسندگان
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