کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1943973 1053169 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane interactions of proline-rich antimicrobial peptide, Chex1-Arg20, multimers
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Membrane interactions of proline-rich antimicrobial peptide, Chex1-Arg20, multimers
چکیده انگلیسی


• Proline-rich antimicrobial peptides (PrAMPs) exhibit different mechanisms in vesicles and live bacteria.
• The designed PrAMP, Chex1-Arg20, and its oligomers did not induce dye leakage in model membranes.
• Chex1-Arg20 oligomers showed stronger binding to anionic phospholipid bilayers and led to liposome aggregation.

The increasing prevalence of antibiotic-resistant pathogens requires the development of new antibiotics. Proline-rich antimicrobial peptides (PrAMPs), including native apidaecins, Bac7, and oncocins or designed A3APO, show multi-modal actions against pathogens together with immunostimulatory activities. The interactions of the designed PrAMP, Chex1-Arg20, and its dimeric and tetrameric oligomers with different model membranes were investigated by circular dichroism spectroscopy, dynamic light scattering, zeta potential, differential scanning calorimetry, and dye leakage. Chex1-Arg20 oligomers showed stronger affinity and preferential binding to negatively charged phospholipid bilayers and led to lipid aggregation and neutralization. Fluorescence microscopy of negatively charged giant unilamellar vesicles with AlexFluor-647-labeled Chex1-Arg20 dimers and tetramers displayed aggregation at a peptide/lipid low ratio of 1:200 and at higher peptide concentrations (1:100/1:50) for Chex1-Arg20 monomer. Such interactions, aggregation, and neutralization of PrAMP oligomers additionally showed the importance of interactions of PrAMPs with negatively charged membranes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes - Volume 1858, Issue 6, June 2016, Pages 1236–1243
نویسندگان
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