Heritability of genetic variants of resistin gene in patients with coronary artery disease: A family-based study

ObjectiveThe resistin gene (RETN) −420 C > G and + 299 G > A polymorphism was investigated in a case-control study from forty complex Pakistani families with coronary artery disease (CAD) history. Heritability of the susceptible/variant alleles was investigated from parent–offspring trios in these families.MethodResistin levels were determined from 239 individuals by enzyme-linked immunosorbent assay. Genotyping was performed using polymerase chain reaction and restriction fragment length polymorphism.ResultsElevated resistin levels were observed from CAD cases vs. controls (p < 0.0001). The RETN − 420 C > G and + 299 G > A polymorphism was more prevalent in cases vs. controls (p < 0.0001). The transmission disequilibrium test revealed a significant association of the − 420 and + 299 polymorphism with CAD (χ2 = 34.4, p < 0.0001 and χ2 = 31.6, p < 0.0001, respectively).ConclusionElevated resistin, and the RETN −420 C > G and + 299 G > A polymorphism may contribute to familial CAD. The − 420 and + 299 variant alleles are transmitted more frequently from parent to affected offspring. This is the first report on the association of the RETN + 299 G > A polymorphism with CAD.