کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
1971787 1060229 2017 8 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Molecular cloning of kisspeptin receptor genes (gpr54-1 and gpr54-2) and their expression profiles in the brain of a tropical damselfish during different gonadal stages
ترجمه فارسی عنوان
شبیه سازی مولکولی (gpr54-1 و gpr54-2) ژن های گیرنده kisspeptin و ویژگی های بروز آنها در مغز یک دوشیزه‌ماهی گرمسیری در مراحل مختلف غدد جنسی
کلمات کلیدی
صخره مرجانی؛ دوشیزهماهی؛ Kisspeptin؛ Real-time PCR؛ شیطان یاقوت کبود؛ استروئیدها جنسیت؛ زرده
Coral reef; Damselfish; Kisspeptin; Real-time PCR; Sapphire devil; Sex steroids; Vitellogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

The kisspeptin receptor (GPR54) mediates neuroendocrine control of kisspeptin in the brain and acts as a gateway for a pulsatile release of hypothalamic gonadotropin-releasing hormone. This study aimed to clone two GPR54 genes (gpr54-1 and gpr54-2) from the brain of the sapphire devil Chrysiptera cyanea, a tropical damselfish, and to study their involvement in reproduction. The partial sequences of the sapphire devil gpr54-1 cDNA (1059 bp) and gpr54-2 cDNA (1098 bp) each had an open reading frame encoding a protein of 353 and 366 amino acids, respectively, both of which had structural features of a G-protein-coupled receptor. The expression of gpr54-1 mRNA was observed in the diencephalon and telencephalon, and gpr54-2 mRNA was found in the optic tectum of sapphire devil. When gpr54-1 and gpr54-2 mRNA levels were examined in the brain of sapphire devil by real-time quantitative polymerase chain reaction (qPCR), they were found to increase during late vitellogenesis and post-spawning. Treatment of fish with estradiol-17β (Ε2) resulted in an increase in gpr54-1 and gpr54-2 expression in the brain of sapphire devil. Thus, kisspeptin receptors likely mediate the activity of kisspeptin in the brain and are involved in controlling reproductive events in a tropical damselfish.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 203, January 2017, Pages 9–16
نویسندگان
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