Transcriptional responses to loss of RNase H2 in Saccharomyces cerevisiae

► Deleting RNH201 affects expression of many genes in budding yeast. ► Deleting RNH201 elicits a strong cellular stress response. ► Deleting RNH201 influences transcription by RNA Polymerases I and III. ► The transcriptional response to deleting RNH201 suggests multiple roles for RNase H2.