کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1980337 | 1061845 | 2012 | 9 صفحه PDF | دانلود رایگان |
Transcription factor II H (TFIIH) is composed of core TFIIH and Cdk-activating kinase (CAK) complexes. Besides transcription, TFIIH also participates in nucleotide excision repair (NER), verifying DNA lesions through its helicase components XPB and XPD. The assembly state of TFIIH is known to be affected by truncation mutations in xeroderma pigmentosum group G/Cockayne syndrome (XP-G/CS). Here, we showed that CAK component MAT1 was rapidly recruited to UV-induced DNA damage sites, co-localizing with core TFIIH component p62, and dispersed from the damage sites upon completion of DNA repair. While the core TFIIH–CAK association remained intact, MAT1 failed to accumulate at DNA damage sites in fibroblasts harboring XP-B or XP-B/CS mutations. Nevertheless, MAT1, XPD and XPC as well as XPG were able to accumulate at damage sites in XP-D fibroblasts, in which the core TFIIH-CAK association also remained intact. Interestingly, XPG recruitment was impaired in XP-B/CS fibroblasts derived from patients with mild phenotype, but persisted in XP-B/CS fibroblasts from severely affected patients resulting in a nonfunctional preincision complex. An examination of steady-state levels of RNA polymerase II (RNAPII) indicated that UV-induced RNAPII phosphorylation was dramatically reduced in XP-B/CS fibroblasts. These results demonstrated that the CAK rapidly disassociates from the core TFIIH upon assembly of nonfunctional preincision complex in XP-B and XP-B/CS cells. The persistency of nonfunctional preincision complex correlates with the severity exhibited by XP-B patients. The results suggest that XPB and XPD helicases differentially regulate the anchoring of CAK to core TFIIH during damage verification step of NER.
► While TFIIH is intact, its CAK component fails to accumulate at DNA damage sites in XP-B and XP-B/CS but not in XP-D cells.
► XPG recruitment is impaired in XP-B/CS cells harboring a F99S mutation but persists in XP-B/CS cells with C-terminal alteration in XPB protein.
► UV-induced RNAPII phosphorylation is dramatically reduced and the RNAPII degradation is delayed in XP-B/CS cells.
► CAK rapidly disassociates from core TFIIH upon assembly of nonfunctional preincision complex in XP-B and XP-B/CS cells.
► The overall work suggests a differential role of XPB and XPD helicases in regulating the anchoring of CAK to core TFIIH during NER.
Journal: DNA Repair - Volume 11, Issue 12, 1 December 2012, Pages 942–950