کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1981146 | 1061902 | 2006 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnesium influences the discrimination and release of ADP by human RAD51
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کلمات کلیدی
ΦX174dsDNAHEPESbacteriophage ϕX174HRAD51DTTNPFssDNATLCRecAADPATPγSSingle-stranded DNA - DNA تک رشته ایdouble-stranded DNA - DNA رشته ایMg2+ - Mg2 +N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] - N- [2-hydroxyethyl] piperazine-N '- [2-ethanesulfonic acid]Adenosine Triphosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPadenosine diphosphate - آدنوزین دی فسفاتEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدHuman Rad51 - انسان رادل 51ATPase - ایتیپیایزها base pair - جفت پایهdithiothreitol - دیتیوتریتولNucleotide - نوکلئوتیدthin layer chromatography - کروماتوگرافی لایه نازکmagnesium ion - یون منیزیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
hRAD51 lacks cooperative DNA-dependent ATPase activity and appears to function with 5-10-fold less Mg2+ compared to RecA. We have further explored the effect of Mg2+ on adenosine nucleotide binding, ATPase, and DNA strand exchange activities. hRAD51 was saturated with the poorly hydrolyzable analog of ATP, ATPγS, at approximately 0.08 mM Mg2+. In contrast, >0.5 mM Mg2+ was required to saturate hRAD51 with ADP. We found ADP to be a significantly less effective competitive inhibitor of the hRAD51 ATPase at low Mg2+ concentrations (0.08 mM). Mg2+ did not appear to affect the ability of ATPγS to competitively inhibit the hRAD51 ATPase. Low Mg2+ (0.08-0.12 mM) enhanced the steady-state ATPase of hRAD51 while higher Mg2+ concentration (>0.3 mM) was inhibitory. At low Mg2+, hRAD51 appeared capable of nearly complete hydrolysis of available ATP, suggesting a lack of ADP product inhibition. There was a strong correlation between the amount of Mg2+ required for stable ADP binding and the inhibition of hRad51 strand exchange activity. Simultaneous inclusion of exogenous ATP and chelation of Mg2+ with EDTA significantly enhanced ADP â ATP exchange by hRAD51. These studies are consistent with the hypothesis that Mg2+ influences the discrimination and release of ADP, which may sequentially impose an important regulatory step in the hRAD51 ATPase cycle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: DNA Repair - Volume 5, Issue 6, 10 June 2006, Pages 704-717
Journal: DNA Repair - Volume 5, Issue 6, 10 June 2006, Pages 704-717
نویسندگان
Kang-Sup Shim, Gregory Tombline, Christopher D. Heinen, Nicole Charbonneau, Christoph Schmutte, Richard Fishel,