کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988733 1540449 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quinolinic acid-immunoreactivity in the naïve mouse brain
ترجمه فارسی عنوان
Quinolinic اسید، رنگپذیری در مغز موش ساده لوحانه
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Brain expression of quinolinic acid (QUIN) in healthy control conditions is unknown.
• QUIN immunoreactivity is found in the cingulate cortex and thalamic reticular nucleus.
• In males, QUIN immunoreactivity in the TRN strongly correlated with locomotion.
• No significant differences between sexes were observed for QUIN immunoreactivity.

Quinolinic acid (QUIN) has been suggested to be involved in infections, inflammatory neurological disorders and in the development of psychiatric disorders. In this view, several studies have been performed to investigate QUIN localization in the brain and its neurotoxic effects. However, evidence is lacking regarding QUIN in healthy, control conditions. The aim of this study was to investigate the region-specific distribution and pattern of QUIN expression in the naïve mouse brain. In addition, possible sex differences in QUIN-immunoreactivity and its link with affect-related behavioural observations were assessed.For this purpose, naïve mice were subjected to the forced swim test (FST) and 20 min open field (OF) testing to measure affect-related behaviour. Afterwards, brains were assessed for QUIN-immunoreactivity. QUIN-immunoreactivity was particularly observed in the cingulate cortex (CC), highlighting clearly delineated cells, and the thalamic reticular nucleus (TRN), showing a more diffuse staining pattern. Subsequently, QUIN-positive cells in the CC were counted, while QUIN-immunoreactivity in the TRN was examined using gray value measurements. No significant differences between sexes were observed for the number of QUIN-positive cells in the CC, neither in levels of QUIN-immunoreactivity in the TRN. A direct correlation was found between QUIN-positive cells in the CC and QUIN-immunoreactivity in the TRN. Moreover, in male mice, a very strong correlation (rsp = .943; p < .01) between QUIN-immunoreactivity at the level of the TRN and motor activity in the OF was observed. Thus, our results suggest that QUIN – detected in the CC and the TRN – may play a role in regulating motor activity in normal conditions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 71, January 2016, Pages 6–12
نویسندگان
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