کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1990155 | 1540706 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Suppression of retinoic acid receptors may contribute to embryonic skeleton hypoplasia in maternal rats with chronic vitamin A deficiency
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کلمات کلیدی
CRBPA/P - A / PRARs - RAR هاSkeletal development - توسعه اسکلتیEmbryogenesis - جنین زاییRetinoic acid - رتینوئیک اسیدcontrol diet - رژیم غذایی کنترلretinoic acid response element - عنصر پاسخ رتینوئیک اسیدanterior/posterior - قبلی / بعدRat - موش صحراییRARE - نادرVitamin A - ویتامینAVAD - چهHox gene - ژن HoxHomeobox genes - ژنهای HomeoboxHox genes - ژنهای HoxVitamin A deficiency - کمبود ویتامین ARetinoic acid receptor - گیرنده اسید رتینوئیکretinoic acid receptors - گیرنده های اسید رتینوئیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vitamin A (VA) is essential for embryonic development and the retinoic acid receptors (RARs) are crucial in mediating the diverse actions of VA in embryogenesis. However, the association between RARs and teratogenicity on skeleton growth and development of vitamin A deficiency (VAD) is not clear. In this present study, weaning female Sprague-Dawley rats were fed purified diets containing graded levels of VA (0, 0.4, 4 IU/g diet) for 70 days before mating, and some of them were supplemented with VA (10 IU/g diet) through pregnancy. Embryos were recovered at embryonic day 19.5 (E19.5) for the analysis of skeleton growth and development and the E12.5 embryos were collected for analysis of select mRNA of RARα, RARβ, RARγ, Hoxa2, Hoxa5 and Hoxa9. Normal gene expressions and morphogenesis were observed in all embryos from group fed 4 IU/g diet. The embryos from group fed VA-free diet showed a comprehensive suppression of all the genes and general fetal resorption. The embryos from group fed 0.4 IU/g diet exhibited a moderate down-regulation on RARβ, RARγ, Hoxa2 and Hoxa5, and the E19.5 fetuses displayed a series of skeletal hypoplasia. The VA supplement groups fed 10 IU/g diet displayed normal gene expressions and morphologic appearances. These findings suggested that the suppression of RARs resulted from VAD could disturb the proper expression of homeobox genes, which might, at least in part, contribute to the embryonic skeletal hypoplasia due to maternal rats with chronic VAD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 21, Issue 8, August 2010, Pages 710-716
Journal: The Journal of Nutritional Biochemistry - Volume 21, Issue 8, August 2010, Pages 710-716
نویسندگان
Na Li, Shanshan Sun, Di Wang, Ping Yao, Xuefeng Yang, Hong Yan, Yukai Du, Chengjiang Ying, Liegang Liu,