کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994399 1064779 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mapping yeast origins of replication via single-stranded DNA detection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Mapping yeast origins of replication via single-stranded DNA detection
چکیده انگلیسی

Studies in th Saccharomyces cerevisiae have provided a framework for understanding how eukaryotic cells replicate their chromosomal DNA to ensure faithful transmission of genetic information to their daughter cells. In particular, S. cerevisiae is the first eukaryote to have its origins of replication mapped on a genomic scale, by three independent groups using three different microarray-based approaches. Here we describe a new technique of origin mapping via detection of single-stranded DNA in yeast. This method not only identified the majority of previously discovered origins, but also detected new ones. We have also shown that this technique can identify origins in Schizosaccharomyces pombe, illustrating the utility of this method for origin mapping in other eukaryotes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 41, Issue 2, February 2007, Pages 151–157
نویسندگان
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