کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994407 1064779 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypothesis testing via integrated computer modeling and digital fluorescence microscopy
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Hypothesis testing via integrated computer modeling and digital fluorescence microscopy
چکیده انگلیسی

Computational modeling has the potential to add an entirely new approach to hypothesis testing in yeast cell biology. Here, we present a method for seamless integration of computational modeling with quantitative digital fluorescence microscopy. This integration is accomplished by developing computational models based on hypotheses for underlying cellular processes that may give rise to experimentally observed fluorescent protein localization patterns. Simulated fluorescence images are generated from the computational models of underlying cellular processes via a “model-convolution” process. These simulated images can then be directly compared to experimental fluorescence images in order to test the model. This method provides a framework for rigorous hypothesis testing in yeast cell biology via integrated mathematical modeling and digital fluorescence microscopy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 41, Issue 2, February 2007, Pages 232–237
نویسندگان
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