کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1994531 1064794 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Application of photobleaching for measuring diffusion of prion proteins in cytosol of yeast cells
چکیده انگلیسی

Measurement of fluorescence recovery after photobleaching (FRAP) is a non-invasive technique for studying protein dynamics in real time in living cells. FRAP studies are carried out on proteins tagged with green fluorescent protein (GFP) or one of its spectral variants. Illumination with high intensity laser light irreversibly bleaches the GFP fluorescence but has no effect on protein function. By photobleaching a limited region of the cytoplasm, the rate of fluorescence recovery provides a measure of the rate of protein diffusion. A detailed description of the FRAP technique is given, including its application to measuring the mobility of GFP-tagged Sup35p in [psi−] and [PSI+] cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 39, Issue 1, May 2006, Pages 43–49
نویسندگان
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