کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2032338 1542867 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis
چکیده انگلیسی

ObjectiveTo investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis.MethodsThis peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed.ResultsNo significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC50 of mast cells degranulation at 126 μmol/L, which was approximately high implying that this peptide had high selectivity for normal cells and did not cause histamine release from these cells.ConclusionsThis would be a great aim in new drug development, in which an agent acts potentially on its target tissue without activating the immune system.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Asian Pacific Journal of Tropical Biomedicine - Volume 6, Issue 3, March 2016, Pages 259–264
نویسندگان
, , , , , ,