کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2039416 1073055 2016 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cortical Polarity of the RING Protein PAR-2 Is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Cortical Polarity of the RING Protein PAR-2 Is Maintained by Exchange Rate Kinetics at the Cortical-Cytoplasmic Boundary
چکیده انگلیسی


• Phosphorylation and oligomerization alter the dynamics of single PAR2 particles in vivo
• Non-linear dynamics are achieved through these different PAR-2 species
• Cortical PAR-2 polarity is reproduced in silico using parameters measured in vivo
• Cortical-cytoplasmic exchange kinetics are critical for PAR-2 polarity

SummaryCell polarity arises through the spatial segregation of polarity regulators. PAR proteins are polarity regulators that localize asymmetrically to two opposing cortical domains. However, it is unclear how the spatially segregated PAR proteins interact to maintain their mutually exclusive partitioning. Here, single-molecule detection analysis in Caenorhabditis elegans embryos reveals that cortical PAR-2 diffuses only short distances, and, as a result, most PAR-2 molecules associate and dissociate from the cortex without crossing into the opposing domain. Our results show that cortical PAR-2 asymmetry is maintained by the local exchange reactions that occur at the cortical-cytoplasmic boundary. Additionally, we demonstrate that local exchange reactions are sufficient to maintain cortical asymmetry in a parameter-free mathematical model. These findings suggest that anterior and posterior PAR proteins primarily interact through the cytoplasmic pool and not via cortical diffusion.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 16, Issue 8, 23 August 2016, Pages 2156–2168
نویسندگان
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