The plastid-dividing machinery: formation, constriction and fission

Plastids divide by constriction of the plastid-dividing (PD) machinery, which encircles the division site. The PD machinery consists of the stromal inner machinery which includes the inner PD and filamenting temperature-sensitive mutant Z (FtsZ) rings and the cytosolic outer machinery which includes the outer PD and dynamin rings. The major constituent of the PD machinery is the outer PD ring, which consists of a bundle of polyglucan filaments. In addition, recent proteomic studies suggest that the PD machinery contains additional proteins that have not been characterized. The PD machinery forms from the inside to the outside of the plastid. The constriction seems to occur by sliding of the polyglucan filaments of the outer PD ring, aided by dynamin. The final fission of the plastid is probably promoted by the ‘pinchase’ activity of dynamin.

► Plastids divide using plastid-dividing machinery during the plastid-division phase.
► The PD machinery consists of stromal inner and cytosolic outer machineries.
► The PD machinery forms from the stromal side to the cytosolic side of the plastid.
► Constriction probably occurs by sliding of the outer PD ring polyglucan filaments, aided by dynamin.
► Final fission of the plastid is probably promoted by the ‘pinchase’ activity of dynamin.