Transfection of insect cell in suspension for efficient baculovirus generation

Baculovirus (BV) mediated insect cell expression system utilizes transfection as a first step to introduce recombinant baculovirus DNA into insect cells. Many labs are still relying on the conventional liposome based transfection method in adherent culture. Here we describe a more efficient method that can replace the existing method. This method is economical and does not require any special adjustment in existing labs.
• An innovative method of transfecting insect cells in suspension using polyethyleneimine (PEI) is described here.
• The beauty of this method is minimal intermediate manipulation of culture during transfection and virus generation.
• The method significantly reduces the chances of cross contamination of viruses while handling multiple targets and constructs as well as the other microbial contamination.

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