کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2072510 1544715 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA methylation and NF-Y regulate Piwil1 expression during chicken spermatogenesis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
DNA methylation and NF-Y regulate Piwil1 expression during chicken spermatogenesis
چکیده انگلیسی


• CCAAT box is necessary for the transcriptional activity of the Piwil1 in Chicken.
• NF-Y binds specifically to the CCAAT box in Piwil1 promoter in chicken germ cells.
• NF-Y and CpG methylation regulate specific expression of Piwil1 in chicken germ cells.

The P-element induced wimpy testis (Piwi) protein family, a subfamily of the Argonaute protein family, is involved in gene silencing and shows specific expression in spermatogenic cells. To reveal the transcriptional regulatory mechanisms of Piwil1 in chickens, we cloned sequences of the chicken Piwil1 promoter region and performed luciferase reporter and electrophoretic mobility shift assays to analyze the transcriptional activity and identify important transcriptional regulatory elements. The results showed that the region from −90 to −43 in the 5′-flanking region of Piwil1 contains a transcriptional regulatory CCAAT box that was necessary for the transcriptional activity of the Piwil1 promoter. Moreover, the transcription factor nuclear factor Y (NF-Y) was bound to the Piwil1 promoter CCAAT box specifically in germ cells. In addition, bisulfite sequencing to determine the methylation profile of the Piwil1 promoter CpG island in different spermatogenic and non-germ cell populations was performed. Compared with germ cells, non-germ cells showed increased methylation of the promoter region containing the CCAAT box, loss of NF-Y binding, and silencing of the Piwil1 locus. It is demonstrated that the specific expression of Piwil1 in chicken germ cells is regulated by the transcription factor NF-Y and differential CpG island methylation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 162, November 2015, Pages 95–103
نویسندگان
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