کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2074771 1544816 2007 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
The effect of cryopreservation on sperm head morphometry in Florida male goat related to sperm freezability
چکیده انگلیسی

The Sperm Class Analyzer was used to investigate the effect of freeze-thawing procedure on Florida buck sperm head morphometry, and to relate possible changes in sperm head dimensions to cryopreservation success.Semen samples (n = 76) were frozen with tris and milk-based extenders and thawed. Sperm quality samples (motility, morphology, acrosome), and sperm head morphometric values (length, width, area, perimeter, ellipticity) were compared between fresh and frozen-thawed samples. Sperm freezability was judged according to the sperm quality parameters assessed. Fertility data was obtained after artificial insemination with cryopreserved semen.Cryopreservation success was different between freezing methods. Sperm head dimensions were significantly (p < 0.05) smaller in cryopreserved tris and milk spermatozoa respectively than in those of the fresh samples. The sperm head morphometric parameters that had changed after cryopreservation were lower in suitable semen samples after thawing and with successful pregnancies after artificial insemination. These data suggest that changes in sperm head morphometry might reflect spermatozoa injury occurred during cryopreservation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 100, Issues 1–2, July 2007, Pages 61–72
نویسندگان
, , ,