Application of agriculture waste as a support for lipase immobilization

Agricultural production contributes considerable waste generating green house gases and impacting environmental quality. This study describes a use for agricultural waste residues as a support for immobilization of Acinetobacter baylyi lipase (ABL), a solvent-stable lipase. Six agricultural waste residues were evaluated for their abundances, morphological characteristics, and immobilization efficiency. Salacca wallichiana stem had the highest immobilization efficiency. Immobilized ABL had a lower optimum temperature compared to the suspended enzyme and showed remarkable solvent stability. Reusability for palm oil hydrolysis of immobilized ABL was comparable to that for commercial lipase, Novozyme 435. Interestingly, ABL immobilized on S. wallichiana stem showed improved storage stability at room temperature (25–32 °C).