Studying the effect of nature of glass surface on immobilization of glucose isomerase

• Immobilization of glucose isomerase on smooth and rough glass beads.
• Immobilization yield is significant.
• Comparison of immobilization of glucose isomerase on smooth and rough glass beads.

Immobilization of glucose isomerase (GI) (EC 5.3.1.5) has been carried out on non-porous glass surfaces using γ-aminopropyltriethoxysilane and polyethyleneimine as an activator and gluteraldehyde as cross linking agent for its potential use. Polyethyleneimine was found to be a superior immobilization activator than γ-aminopropyltriethoxysilane. Further, the effects of rough and smooth surfaces of non-porous glass beads on immobilization of glucose isomerase have been investigated. Polyethyleneimine on rough glass beads was found to be better activator material than that of γ-aminopropyltriethoxysilane. The prime objective of study was to immobilize glucose isomerase on an improved surface of non-porous glass beads which will give a significantly higher immobilization yield. After optimizing the parameters the immobilization yield increased from an initial value 29.43% to 79.6%.

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