Ames positive boronic acids are not all eukaryotic genotoxins

• O’Donovan reported positive Ames test results for 9 Boronic acids.
• One compound was positive in GreenScreen HC and 4 in BlueScreen HC.
• Positive GADD45a data were produced only at concentrations above 1 mM.
• No compounds produced positive results in the presence of S9.

Boronic acids and their derivatives have been exploited for their pharmacological activity and their utility as intermediates in the synthesis of novel non-boron containing compounds. A recent study reported that boronic acids are bacterial mutagens. Here, results are reported from the testing of nine boronic acids using the pan-mechanistic eukaryotic GADD45a genotoxicity assays, BlueScreen HC and GreenScreen HC. Positive results were produced for one compound in GreenScreen and four compounds in BlueScreen. Only negative results were produced when tested with S9 metabolic activation. These data suggest that there is not a general genotoxic liability in eukaryotes, within this chemical domain. Furthermore, they are not potent eukaryotic genotoxins: positive results were produced only at concentrations between 1 mM and 10 mM. Their presence as low concentration contaminants or impurities would be unlikely to produce misleading positive results for a test material.