Ex vivo aorta patch model for analysis of cellular adhesion

• Here we present a new model to study cell–endothelium interaction onto an intact endothelial cell layer by plating HCT-116 cells onto mouse aortic patches.
• Validation of this model was performed by demonstrating changes in cell shape and development of cell protrusions typical for cell spreading.
• A major advantage of this model is that cell–endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.

The vascular endothelium as well as subendothelium are objects of many researches as it is directly involved in a multiplicity of physiological and pathological settings. Detailed study of endothelial function became feasible with the development of techniques to culture endothelial cells (EC) in vitro. Limitations of this approach have become apparent with the realization that cell culture dedifferentiate with time and do not exhibit properties of intact tissue. Here we describe the development of a novel ex vivo tissue model to study cell–vascular wall interactions by using isolated mouse aorta patches. Validation of this model was performed by demonstrating cell attachment and changes in cell shape typical for cell spreading during adhesion. A major advantage of this model is that cell–endothelium interaction and its molecular backgrounds can now be studied more feasibly on an intact and native tissue.