کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2409633 | 1103225 | 2017 | 10 صفحه PDF | دانلود رایگان |
The role of poly(d,l-lactic-co-glycolic acid, PLGA) microparticles on enhancing immune responses of multiepitope DNA vaccines was investigated in vitro and in vivo. pcDNA-SG encoding T and B cell epitopes of foot-and-mouth disease virus (FMDV) was encapsulated into PLGA microparticles. PLGA microparticles could protect themselves from nuclease degradation in vitro. PLGA–pcDNA-SG microparticles could be uptaken by cells and expressed His-tagged SG immunogen in vitro and in vivo. A prolonged expression and presentation of SG immunogen were observed by confocal laser scanning microscopy in the lymphocytes from the mice incubated with PLGA–pcDNA-SG microparticles, compared with the mice immunized with naked pcDNA-SG. PLGA–pcDNA-SG microparticles displayed a significant stronger immunogenicity than naked DNA vaccines with a higher titer of virus-specific antibody, elevated IFN-γ production and enhanced lymphocyte proliferation. PLGA–DNA microparticle could elicit augmented humoral and cellular responses with reduced amounts and times of immunization.
Journal: Vaccine - Volume 24, Issue 12, 15 March 2006, Pages 2017–2026