کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429212 | 1106484 | 2014 | 11 صفحه PDF | دانلود رایگان |
• Phenotypically distinct CD205Hi and CD205Lo myeloid DC subpopulations are present in bovine blood.
• CD205Hi and CD205Lo myeloid DCs have an equal capacity to acquire antigen.
• CD205Lo but not CD205Hi myeloid DCs efficiently activate T cell.
• CD205Lo and CD205Hi myeloid DCs may represent developmentally distinct DC lineages.
Immature myeloid (m)DCs circulating in the blood of cattle have been defined as lineage negative (Lin−)MHCII+CD11c+CD205+ cells. Lin−MHCII+CD11c+CD205+ mDCs (0.2% blood mononuclear cells) isolated from bovine blood were heterogeneous in cell size and CD205 expression. Using highspeed cell sorting, Lin−MHCII+CD11c+CD205+ DCs were sorted into CD205Hi and CD205Lo subpopulations which were phenotypically distinct and differed significantly (P < 0.01) in TLR gene expression. CD205Hi and CD205Lo mDCs were more efficient in macropinocytosis than monocytes and expressed no or little detectable non-specific esterase activity. CD205Lo mDCs efficiently activated purified allogeneic T cells and the addition of TLR agonists did not significantly alter this antigen presentation capacity. T cell activation by CD205Lo mDCs was associated with differential up-regulation of CD40, CD80, CD86 and TGFβ1 gene expression when compared to CD205Hi mDCs. In conclusion, two phenotypically and functionally distinct CD11c+CD205+ mDCs were isolated from blood that had an equal capacity to acquire antigen but markedly different capacities to activate T cells.
Journal: Developmental & Comparative Immunology - Volume 44, Issue 2, June 2014, Pages 378–388