Optimisation for high cell density cultivation of Lactobacillus salivarius BBE 09-18 with response surface methodology

Based on the nutritional and physiological requirements of lactic acid bacteria (LAB), the effects of different carbon sources, nitrogen sources, buffer salts, microelements and growth factors on the growth of Lactobacillus salivarius BBE 09-18 (L. salivarius BBE 09-18) were investigated, among which three key factors were determined and optimised by response surface methodology. Estimated optimal conditions of the factors for the growth of L. salivarius BBE 09-18 determined. After cultivated in this optimised medium for 18 h at 37 °C, the viable cell count for L. salivarius BBE 09-18 was 5.94 × 109 cfu mL−1, which was about 3 times higher than that obtained in standard Man Rogosa Sharpe medium. This study will lay a solid foundation for the high cell density cultivation of L. salivarius BBE 09-18 to circumvent bottlenecks encountered in the production of LAB starters and probiotic ingredients in the field of dairy.