کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2507741 1117505 2015 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quality evaluation of synthetic quorum sensing peptides used in R&D
ترجمه فارسی عنوان
ارزیابی کیفی پپتیدهای سنجش قوام سنتتیک مورد استفاده در تحقیق و توسعه
کلمات کلیدی
پپتیدهای حساس به قاعده، کیفیت، پروفایل نامطلوب
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
چکیده انگلیسی

Peptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in R&D and therefore a representative set of quorum sensing peptides, with a requested purity of at least 95.0%, was evaluated for their purity and nature of related impurities. In-house quality control (QC) revealed a large discrepancy between the purity levels as stated on the supplier׳s certificate of analysis and our QC results. By using our QC analysis flowchart, we demonstrated that only 44.0% of the peptides met the required purity. The main compound of one sample was even found to have a different structure compared to the desired peptide. We also found that the majority of the related impurities were lacking amino acid(s) in the desired peptide sequence. Relying on the certificates of analysis as provided by the supplier might have serious consequences for peptide research, and peptide-researchers should implement and maintain a thorough in-house QC.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Analysis - Volume 5, Issue 3, June 2015, Pages 169–181
نویسندگان
, , , , ,