کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2509228 1117654 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Separation and purification and in vitro anti-proliferative activity of leukemia cell K562 of Galium aparine L. petroleum ether phase
ترجمه فارسی عنوان
جداسازی و تصفیه و فعالیت ضدتکثیر سلول K562 لوسمی از گلومیرریکین فاز اتر فاز پتروشیمی L. petroleum
کلمات کلیدی
Galium aparine L .؛ تصفیه؛ K562؛ MTT
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی علوم دارویی
چکیده انگلیسی

To explore material basis of in vitro anti-proliferative activity of leukemia cell K562 of petroleum ether phase of product resulting from Galium aparine L. 60% ethanol extraction, the experiment adopts column chromatography combined with thin layer preparation, isolates and purifies petroleum ether, conducts structural identification of obtained single compound and applies MTT method for viability assay of in vitro anti-proliferative activity of leukemia cell K562. Experimental results show that G. aparine L. petroleum ether contains mainly β-sitosterol, daucosterol and dibutyl phthalate and other substances. Under experimental conditions, the three could inhibit the proliferation of leukemia cell K562 with dose-effect and time-effect relationship, of which dibutyl phthalate has strongest activity. Dibutyl phthalate with excellent activity, β-sitosterol with rich content and moderate effect should be the main contributor to its biological activity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Pharmaceutical Journal - Volume 24, Issue 3, May 2016, Pages 241–244
نویسندگان
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