کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2524464 | 1119572 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cisplatin upregulates MSH2 expression by reducing miR-21 to inhibit A549 cell growth
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کلمات کلیدی
human MutL homolog 1TPM1FACShMSH2miRNAsTropomyosin 1RT-PCRhMLH1MSH2MMR3′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشدهGene expression - بیان ژنmismatch repair - تعمیر ناسازگاریmicroRNAs - ریز آرانایLung cancer - سرطان ریهcisplatin - سیس پلاتینFlow cytometry - فلوسیتومتریMicroRNA - میکرو RNA Nucleotides - نوکلئوتیدreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوس
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
miR-21 can act as an oncogene. MSH2 has been reported that it involved in the DNA mismatch repair (MMR) system and overexpression of MSH2 can induce cell apoptosis. We predicted that MSH2-3â²-untranslated region (3â²-UTR) was targeted by miR-21 using microRNA analysis softwares. To further explore the roles of miR-21 and MSH2 in A549 cells, we constructed pcDNA-GFP-msh-UTR vector (including MSH2-3â²-UTR) to transfect A549 cells with miR-21, GFP positive cells were estimated under a fluorescence microscopy and by flow cytometry. We found miR-21 could obviously downregulate the expression of MSH2, which was further proved by western blotting. Moreover, we treated A549 cells with cisplatin and found that cisplatin could inhibit A549 cell growth in vitro and in vivo. We also found that cisplatin could downregulate miR-21 expression, while increase MSH2 expression in A549 cells. Our results demonstrated that cisplatin could upregulate the expression of MSH2 through downregulating miR-21 to inhibit A549 cell proliferation, which provides new gene targets for drug design or cancer therary.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 67, Issue 2, March 2013, Pages 97-102
Journal: Biomedicine & Pharmacotherapy - Volume 67, Issue 2, March 2013, Pages 97-102
نویسندگان
Yan-Xia Zhang, Zhen Yue, Ping-Yu Wang, You-Jie Li, Jia-Xuan Xin, Min Pang, Qing-Yin Zheng, Shu-Yang Xie,