Acanthopanax senticosus exerts neuroprotective effects through HO-1 signaling in hippocampal and microglial cells

Extracts of Acanthopanax senticosus, a traditional herb commonly found in Northeastern Asia, are used for treating neurodegenerative disorders such as ischemia and depression. However, the mechanisms of its neuroinflammatory and cytoprotective effects have not been investigated. We examined the mechanism of A. senticosus activity in anti-neuroinflammatory and neuroprotective processes. HO-1 is an inducible enzyme present in most cell lines. ASE increased HO-1 expression, which reduced LPS-induced nitric oxide/ROS production in BV2 cells. Moreover, the induction of HO-1 expression protected cells against glutamate-induced neuronal cell death. Activation of the p38-CREB pathway and translocation of Nrf2 are strongly involved in ASE-induced HO-1 expression. Our results showed that ASE-induced HO-1 expression through the p38-CREB pathway plays an important role in the generation of anti-neuroinflammatory and neuroprotective responses. ASE also increases the translocation of Nrf2 to regulate HO-1 expression. Furthermore, our results indicate that ASE serves as a potential therapeutic agent for neuronal disorders.

► ASE is a traditional medicine used in East Asia.
► ASE possesses anti-inflammatory and neuroprotective effects via HO-1 signal.
► ASE's cytoprotective effect in glutamate-induced hippocampal HT22 cells.
► Its anti-neuroinflammatory effects in LPS-stimulated microglial BV2 cells.
► HO-1 signal induction by ASE via the p38-CREB and Nrf2 pathways.