Nitrous oxide related behavioral and histopathological changes may be related to oxidative stress

• One-month exposure of N2O resulted in behavioral, biochemical and histopathological changes in rat.
• GSH, TAC, vitamin B12 level correlate with spontaneous locomotor activity and grip strength.
• N2O exposure is associated with myelin loss in cerebral cortex, spinal cord and high serum HCY level.
• N2O related changes may be due to interference with cobalamin metabolism and oxidative stress.

Nitrous oxide (N2O) toxicity can result in myelin loss and hyperhomocysteinemia similar to cobalamin (Cbl) deficiency. Studies on N2O exposure can help in understanding the mechanism of demyelination. In view of paucity of studies on N2O toxicity in rats this study was undertaken. Six male wistar rats were exposed to 1.5 L/min N2O with 1:1 O2 for 90 min daily for 1 month. After 1-month exposure blood homocysteine (HCY) and oxidative stress parameters glutathione (GSH) and total antioxidant capacity (TAC) were measured. Brain and spinal cord was subjected to histopathological examination. The neurobehavioral changes, oxidative stress parameters and histopathological changes were correlated with serum B12 and HCY level. After 1-month exposure, the rats appeared sluggish, lethargic and developed predominantly hind limb weakness for 1–1.5 h. In the exposed group, the total distance traveled (2001.66 ± 118.27 cm; p = 0.037), time moving (80.16 ± 5.7 s; p = 0.028), number of rearing (10.33 ± 1.45; p = 0.014) and grip strength (1042.40 ± 51.3 N; p = 0.041) were significantly decreased whereas, resting time significantly increased (219.83 ± 5.7 s; p = 0.030) compared to controls. Serum HCY level was significantly increased (20.56 ± 1.296 μm/ml; p = 0.0007) in the exposed group. However, serum B12 and folic acid levels were not significantly different. GSH significantly decreased (2.21 ± 0.60 mg/dl; p = 0.018) along with TAC (0.76 ± 0.16 Trolox_Eq_mmol/l; p = 0.036). The histopathological studies revealed shrinkage and vacuolation of neurons in cerebral cortex, focal myelin loss, vacuolation in subcortical white matter and spinal cord. N2O exposure results in behavioral alterations, hyperhomocysteinemia, cortical and spinal cord demyelination which were associated with decrease GSH and TAC highlighting pathophysiological role of oxidative stress.

N2O exposure of 1.5 L/min for 90 min for 1 month results in myelin loss in spinal cord and subcortical white mater.Figure optionsDownload high-quality image (213 K)Download as PowerPoint slide