کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2603096 | 1133808 | 2008 | 8 صفحه PDF | دانلود رایگان |
Hexabromocyclododecanes (HBCDs) are additive brominated flame retardants mainly used in plastics and textiles. At the present time, these compounds are found in almost all environmental and human samples. In order to evaluate the environmental safety and health risk of HBCDs, the enantiomerically pure α-, β-, and γ-HBCD were prepared using high performance liquid chromatography (HPLC) on a PM-β-CD column and the cytotoxicities of their enantiomers were evaluated in Hep G2 cells. Results from the 3-(4,5-dimethylthioazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), resazurin reduction and lactate dehydrogenase (LDH) release assays showed a good agreement that the order of cytotoxicity was γ-HBCD ⩾ β-HBCD > α-HBCD, and that significantly lower cell viability and higher LDH release were observed in all (+)-enantiomers ((+) α-, (+) β- and (+) γ-HBCD) than the corresponding (−)-forms ((−) α-, (−) β- and (−) γ-HBCD). Additionally, the formation of reactive oxygen species (ROS) induced by these HBCD enantiomers were detected. The positive correlation between the LDH release and ROS formation demonstrated that the toxic mechanism might be mediated by oxidative damage. These results suggest that environmental and human health risks of HBCDs must be evaluated at the level of individual enantiomers.
Journal: Toxicology in Vitro - Volume 22, Issue 6, September 2008, Pages 1520–1527