کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2788061 | 1154379 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
In order to transfer useful genes of Hordeum californicum into common wheat (Triticum aestivum L.), the T. aestivum c.v. Chinese Spring (CS)-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morphological observation, cytological, biochemical and molecular marker techniques. Alien addition lines with two H. californicum chromosomes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indicated that chromosome H3 of H. californicum belongs to homoeologous group 2, and was thus designated 2H. SDS-PAGE showed that chromosome H2 of H. californicum belongs to homoeologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines (DA2H and MA5H) identified were evaluated for powdery mildew (Erysiphe graminis f. sp. triticii) resistance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chromosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetics and Genomics - Volume 35, Issue 11, November 2008, Pages 673-678
Journal: Journal of Genetics and Genomics - Volume 35, Issue 11, November 2008, Pages 673-678
نویسندگان
Fang Kong, Haiyan Wang, Aizhong Cao, Bi Qin, Jianhui Ji, Suling Wang, Xiu-E Wang,