کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2788390 1568568 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Impact of maternal diabetes type 1 on proliferative potential, differentiation and apoptotic activity in villous capillaries of term placenta
ترجمه فارسی عنوان
تأثیر دیابت مادرزادی نوع یک بر پتانسیل پرولیفراتیو، تمایز و فعالیت آپوپتوز در مویرگ‌های پرزدار جفت ترم
کلمات کلیدی
کاسپاز 3؛ کورنگیوز؛ دیابت؛ Ki67؛ مارکر nestin؛ جفت
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• The proliferation, maturation and apoptosis in villous capillaries was compared between normal and diabetic placenta.
• Maternal diabetes mellitus is associated with lower proliferative ability and delayed differentiation of capillaries.
• The placenta in maternal diabetes displays lower potential to comply fetal requirements in the final stage of pregnancy.

IntroductionMaternal diabetes mellitus changes morphology and impairs function of placental capillaries. Here, quantitative parameters characterizing cell proliferation using detection of Ki67, differentiation reflected by nestin expression and apoptosis in placental capillary bed with active caspase 3 as a marker were compared in normal term placentas and placentas from pregnancies complicated by Type 1 maternal diabetes mellitus.MethodsSpecimens of sixteen diabetic placentas and eight control placentas were collected by systematic uniform random sampling. Immunohistochemical detections of Ki67, nestin, and active caspase 3 were performed in histological sections of five haphazardly chosen blocks per placenta. Twenty fields of view per section, i.e. one hundred fields of view per placenta, were used for analysis of proliferation as well as of apoptosis, and in approximately 70 capillary cross-sections per placenta the nestin-positive segments of their circumference were measured.ResultsThe percentage of Ki67-positive cells counted in the capillary wall was significantly lower in diabetic group. The counts of Ki67-labelled nuclei per villous area unit were significantly lower in cytotrophoblast and capillary wall of terminal villi in diabetic placenta. The proportion of nestin-labeled segments of capillary circumference was significantly higher in placentas of diabetic group. No differences in the numbers of apoptotic cells were found between studied groups.DiscussionThe results show that the term placenta in Type 1 diabetes has lower potential to enlarge the surface area of structures involved in maternofetal transport, and that the villous capillary bed displays delayed differentiation. Those factors may participate in decreased ability of diabetic placenta to comply with fetal requirements in the final stage of pregnancy.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 40, April 2016, Pages 1–7
نویسندگان
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