کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2788407 1568567 2016 8 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
Curcumin improves LPS-induced preeclampsia-like phenotype in rat by inhibiting the TLR4 signaling pathway
ترجمه فارسی عنوان
كركومين، فنوتيپ پره اكلامپسي مانند ناشي از LPS را در موش صحرايي با مهار مسیر سیگنالینگ TLR4 بهبود مي دهد
کلمات کلیدی
پره اکلامپسی؛ گیرنده سفارشی 4؛ لیپوپلی ساکارید؛ کرکومین؛ بازسازی سرخرگ شريان
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی


• Curcumin ameliorated the hypertension and proteinuria of LPS-induced PE-like rats.
• Curcumin reversed LPS-induced shallow placental implantation.
• Curcumin prevented the LPS-induced PE-like rats from the renal injury.
• Curcumin suppressed overactivation of TLR4 signaling pathway in PE.
• Curcumin decreased excessive expression of inflammatory cytokines in PE.

IntroductionAbnormal inflammation mediated by Toll-like receptor 4 (TLR4) signaling pathway contributes to preeclampsia (PE). Because curcumin can inhibit TLR4 signaling pathway, we investigated its effects on a PE rat model.MethodsTwenty-one pregnant rats were randomly divided into three groups: 1) seven rats were injected 0.5 μg/kg lipopolysaccharide (LPS) on gestational day (GD) 5 to create a PE model (LPS-treated group), 2) seven rats were injected with a similar dosage of LPS and further treated with curcumin (0.36 mg/kg) (LPS-curcumin-treated group), 3) seven rats received saline (control group). Blood pressure and urinary protein level were observed. Immunostaining and periodic acid-Schiff staining of placenta were conducted. TLR4 and downstream Nuclear Factor-κB (NF-κB) expressions of placenta were analyzed by Western blot and immunohistochemistry. IL-6 and MCP-1 in rat serum and placenta were determined by ELISA and qRT-PCR.ResultsCompared to LPS-treated group, LPS-curcumin-treated group had decreased blood pressure and urinary protein level, similar to control group. Furthermore, deficient trophoblast invasion and spiral artery remodeling induced by LPS were improved by curcumin. Increased TLR4, NF-κB and IL-6, MCP-1 protein expressions in LPS-treated group were significantly decreased after curcumin administration.DiscussionCurcumin improves the PE-like phenotype in rat model by reducing abnormal inflammation related to TLR4 signaling pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 41, May 2016, Pages 45–52
نویسندگان
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