کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2798001 | 1155674 | 2008 | 6 صفحه PDF | دانلود رایگان |
We investigated the effects of fasting on gene expression and intracellular signals regulating energy metabolism in adipose tissue. Following fasting for 15 h or 39 h, epididymal fat pads were isolated from Wistar rats. PPARγ mRNA levels decreased in the adipose tissues isolated from rats fasted for 39 h, whereas adipocyte lipid-binding protein (aP2) and lipoprotein lipase (LPL) mRNA levels increased. Overnight fasting increased the AMP/ATP ratio and AMP-activated protein kinase (AMPK) in adipose tissue, but not in muscle or liver tissue. In addition, the effect of 5-aminoimidazole-4-carboxyamide-ribonucleoside (AICAR) on PPARγ expression in primary cultured adipocytes was investigated. AICAR reduced PPARγ mRNA levels but increased aP2 and LPL mRNA levels. Thus, fasting-induced AMPK activation may affect on the regulation of gene expression in adipocytes.
Journal: Diabetes Research and Clinical Practice - Volume 81, Issue 2, August 2008, Pages 144–149