کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2798272 1155685 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Attenuation of aldose reductase gene suppresses high-glucose-induced apoptosis and oxidative stress in rat lens epithelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
پیش نمایش صفحه اول مقاله
Attenuation of aldose reductase gene suppresses high-glucose-induced apoptosis and oxidative stress in rat lens epithelial cells
چکیده انگلیسی

AimsA major contributory factor to diabetic cataract formation is increased aldose reductase (AR) activity in the polyol pathway. We investigated the effects of aldose reductase inhibition by RNA interference (RNAi) of the aldose reductase gene and administration of an aldose reductase inhibitor (ARI) on the changes induced by high glucose levels in rat lens epithelial cells (RLECs).MethodsSmall interfering RNAs (siRNAs) were designed to target the coding sequence of rat AR-siRNA. RLECs were cultured in either normal or high d-glucose. Western analysis was performed to monitor AR expression. MTS (3-(4-5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt) and TUNEL assays were used to detect apoptotic cell death. Intracellular reactive oxygen species (ROS) were assessed by using DCFH-DA. Activation of nuclear factor-κB (NF-κB) was measured by an ELISA-based detection method.ResultsBoth siRNA and ARI suppressed increased levels of ROS, activation of NF-κB, and apoptotic cell death induced by high glucose levels. Inhibition of rAR expression by siRNA and inhibition of AR activity by ARI also suppressed sorbitol accumulation.ConclusionsBoth inhibition of rAR expression by rAR siRNA and inhibition of rAR activity by an ARI appeared effective in diminishing the changes of RLECs associated with high glucose levels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Diabetes Research and Clinical Practice - Volume 82, Issue 1, October 2008, Pages 18–24
نویسندگان
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