Extra-pituitary prolactin (PRL) and prolactin-like protein (PRL-L) in chickens and zebrafish

• PRL is expressed in the extra-pituitary tissues of chickens and zebrafish.
• PRL-L (PRL2) is a novel functional ligand of PRLR in chickens and zebrafish.
• PRL-L is widely expressed in extrapituitary tissues of chickens and zebrafish.

It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior. However, there is increasing evidence showing that PRL and the newly identified ‘prolactin-like protein (PRL-L)’, a novel ligand of PRL receptor, are also expressed in a variety of extra-pituitary tissues, such as the brain, skin, ovary, and testes in non-mammalian vertebrates. In this brief review, we summarize the recent research progress on the structure, biological activities, and extra-pituitary expression of PRL and PRL-L in chickens (Gallus gallus) and zebrafish (Danio rerio) from our and other laboratories and briefly discuss their potential paracrine/autocrine roles in non-mammalian vertebrates, which may promote us to rethink the broad spectrum of PRL actions previously attributed to pituitary PRL only.