Annual and periovulatory changes in tyrosine hydroxylase activity in the ovary of the catfish Heteropneustes fossilis

In the present study, tyrosine hydroxylase (TH, the rate-limiting enzyme in catecholamine synthesis) activity was demonstrated in the ovary of the catfish to elucidate the possible physiological role of catecholamines in the gonad. The ovary is innervated by seven pairs of nerves, originating from the paired sympathetic chain lying dorsal to the posterior kidney. Ovarian TH activity showed a significant annual variation (P < 0.001, one-way ANOVA), correlating with gonado-somatic index. Activity was low from December to February (resting phase), increased from March to July (recrudescent phase) and then decreased in post-spawning phase (August–November). The annual pattern was similar to that of the brain. An intraperitoneal injection of 100 IU hCG/fish induced significant periovulatory changes in TH activity with the peak rise at 16 h, and the activity decreased after egg-stripping (P < 0.001, one-way ANOVA). Brain TH activity showed similar periovulatory changes. The results suggest that catecholamine synthesis is increased during both ovarian recrudescence and spawning of the annual reproductive cycle, implying a functional role in ovarian growth, maturation and ovulation.