کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2807947 1569066 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro toxicity of the galanin receptor 3 antagonist SNAP 37889
ترجمه فارسی عنوان
سميت در شرایط آزمایشگاهی SNAP 37889 آنتاگونيست گیرنده گالنين
کلمات کلیدی
گالانیان؛ گیرنده گالانین 3؛ SNAP 37889؛ سمیت ؛ اضطراب؛ افسردگی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


• SNAP 37889 produces toxic effects in vitro.
• The toxicity of SNAP 37889 does not depend on endogenous expression of galanin receptors.
• Immune cells are sensitive to SNAP 37889-induced cell death.

Galanin and its receptors (GAL1, GAL2, GAL3) modulate a range of neuronal, immune and vascular activities. In vivo administration of SNAP 37889 (1-phenyl-3-[[3-(trifluoromethyl)phenyl]imino]-1H-indol-2-one), a potent small non-peptidergic antagonist of GAL3, was reported to reduce anxiety- and depression-related behavior, ethanol consumption, and antagonizes the effect of galanin on plasma extravasation in rodent models. Accordingly, SNAP 37889 has been proposed as a potential therapeutic agent to treat anxiety and depression disorders. Therefore, we evaluated the toxicity of SNAP 37889 to different cell types.Our experiments revealed that SNAP 37889 (≥ 10 μM) induced apoptosis in epithelial (HMCB) and microglial (BV-2) cell lines expressing endogenous GAL3, in peripheral blood mononuclear cells and promyelocytic leukemia cells (HL-60) expressing GAL2, and in a neuronal cell line (SH-SY5Y) lacking galanin receptor expression altogether.In conclusion, SNAP 37889 is toxic to a variety of cell types independent of GAL3 expression. We caution that the clinical use of SNAP 37889 at doses that might be used to treat anxiety- or depression- related diseases could have unexpected non-galanin receptor-mediated toxicity, especially on immune cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropeptides - Volume 56, April 2016, Pages 83–88
نویسندگان
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