کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2815388 1159867 2015 19 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Unique synteny and alternate splicing of the chitin synthases in closely related heliothine moths
ترجمه فارسی عنوان
ترکیب مناسبی و ترکیب متناوب سیتاات کیتین در پروتئین های متمایز هلیوتیک
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
چکیده انگلیسی


• Two chitin synthase genes were characterized in the genomes of two heliothine moths.
• In both moths, the genes had a same-strand tandem-orientation with ChSB 5′ of ChSA.
• Transcripts for both HzChSA and HzChSB were alternatively spliced in Helicoverpa zea.
• HzChSB–E12b alternate spliced transcript is the first report of splicing in ChSB group.

Chitin is an extracellular biopolymer that contributes to the cuticular structural matrix in arthropods. As a consequence of its rigid structure, the chitinous cuticle must be shed and replaced to accommodate growth. Two chitin synthase genes that encode for chitin synthase A (ChSA), which produces cuticular exoskeleton, and chitin synthase B (ChSB), which produces peritrophic membrane, were characterized in the genomes of two heliothine moths: the corn earworm/cotton bollworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae) and the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae). In both moths, the two genes were arranged in tandem with the same orientation on the same strand with ChSB located 5′ of ChSA. Sequence comparisons showed that the coding sequences were highly conserved with homologues from other species but that the tandem juxtaposed genomic arrangement of the two genes was unique in these insects. The mechanism that has led to this arrangement is unclear but is most likely a recent recombinational event. Transcript mapping of HzChSB and HzChSA in H. zea demonstrated that both transcripts were differentially spliced in various tissues and larval stages. The identification of the HzChSB–E12b alternate spliced transcript is the first report of alternate splicing for the ChSB group. The importance of this splice form is not clear because the protein produced would lack any enzymatic activity but retain the membrane insertion motifs. As for other insects, these genes provide an important target for potential control through RNAi but also provide a subject for broad scale genomic recombinational events.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 574, Issue 1, 10 December 2015, Pages 121–139
نویسندگان
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