کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2815439 | 1159871 | 2016 | 10 صفحه PDF | دانلود رایگان |
• Ig genes were expressed in umbilical cord blood CD34+ HSC/HPCs.
• IgM was expressed in HSC/HPCs at the highest frequency and level.
• Ig gene expression in HSC/HPCs displayed restricted/biased VHDJH patterns.
Recently, immunoglobulin (Ig) expression was reported in a variety of non-B lineage cells, including myeloid cells. We assessed whether hematopoietic stem/progenitor cells (HSC/HPCs) can express Ig. With Gene Expression Omnibus (GEO) microarray database analysis, we found that IGHM was expressed with the highest frequency and level in umbilical cord blood CD34+ HSC/HPCs, followed by IGK@, IGHE, IGHD, IGHG1, and IGHA1, while IGL@ was nearly not expressed. Ig expression was further confirmed by molecular experiments and immunofluorescence. Moreover, HSC/HPCs-derived Ig displayed restricted/biased usages and VHDJH rearrangement patterns. These results suggest that Igs, especially IgM, may have a role in CD34+ HSC/HPCs function.
Journal: Gene - Volume 575, Issue 1, 1 January 2016, Pages 108–117