کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2815522 | 1159874 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and analysis of HSP70 from Sepiella maindroni under stress of Vibrio harveyi and Cd2Â +
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کلمات کلیدی
ORFhsp70HSPsUTRsVibrio infectionSepiella maindroniCSt - CSTrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدCritical solution temperature - دمای محلول بحرانیopen reading frame - قاب خواندن بازRace - مسابقهUntranslated regions - مناطق غیر ترجمهheat shock protein 70 - پروتئین شوک حرارت 70Heat shock protein 70 (hsp70) - پروتئین شوک حرارت 70 (hsp70)Heat shock proteins - پروتئینهای شوک حرارتی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The 70-kDa heat shock proteins (HSP70) play crucial roles in protecting cells against environmental stresses, such as heat shock, heavy metals and pathogenic bacteria. The full-length HSP70 cDNA of Sepiella maindroni (designated as SmHSP70, GenBank accession no. KJ739788) was 2109Â bp, including an ORF of 1950Â bp encoding a polypeptide of 649 amino acids with predicted pI/MW 5.24/71.30Â kDa, a 62Â bp-5â²-UTR and a 97Â bp-3â²-UTR. BLASTp analysis and phylogenetic relationship strongly suggested that the amino acid sequence was a member of HSP70 family. Multiple sequence alignment revealed that SmHSP70 and other known HSP70 were highly conserved, especially in the regions of HSP70 family signatures, the bipartite nuclear targeting sequence, ATP/GTP-binding site motif and 'EEVD' motif. Time-dependent mRNA expression of SmHSP70 in the liver was recorded by quantitative real-time RT-PCR after Vibrio harveyi injection and Cd2Â + exposure. The results indicated that SmHSP70 played a significant role in mediating the environmental stress and immune response against pathogens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 572, Issue 1, 1 November 2015, Pages 146-152
Journal: Gene - Volume 572, Issue 1, 1 November 2015, Pages 146-152
نویسندگان
Hui-hui Liu, Jian-yu He, Chang-feng Chi, Zhen-ming Lv,